The role of cue salience in prospective memory commission errors in nonperformed nonfocal tasks.

Prospective memory (PM) refers to the ability to remember to execute an intention in the future without having a permanent reminder. These intentions can be performed when they are not relevant or become no-longer needed, the so-called "commission errors". The present study aims to understand the effect of cue salience on PM commission errors with unperformed intentions and on the ongoing task performance-associated costs. Through a between-subjects design, eighty-one participants were assigned to 3 conditions: the no-PM condition, which served as control, and the salient and nonsalient conditions, which were asked to perform a lexical decision task and an incomplete nonfocal prospective memory task (i.e. no PM cues were presented). Subsequently, participants were instructed to no longer execute the prospective intention. In the second phase, a lexical decision task occurred again, including irrelevant PM cues, which should not be answered as such. In the salient condition, cues were salient (i.e. presented in red or blue background). In contrast, in the nonsalient condition, PM cues appeared on a black background, as any other stimuli. In the no-PM control condition, participants only performed an LDT. A commission error occurred when the (irrelevant) intention was performed in this second phase. Results showed that more participants performed a commission error in the presence of salient cues, even when PM intentions became irrelevant. Additionally, when cues were not salient, participants took longer to answer the LDT, as reasoned by the spontaneous retrieval theory. These findings are discussed according to the dual-mechanism account.

Phylogeny and ultrastructure of Myxobolus rangeli n. sp. (Myxozoa, Bivalvulida), a histozoic parasite in Siluriformes fish from the Amazon region.

A new species of Myxobolus parasitizing the arterial bulb and cardiac musculature of the freshwater fish Pimelodus ornatus Kner, 1858, from the Arari river in the municipality of Cachoeira do Arari, island of Marajó, Pará, Brazil, was described. In the present study, the observed prevalence of myxozoan parasites in the heart tissue of the hosts was 20% (6/30). The myxozoans observed had mature biconvex spores, slightly rounded, an anterior end with two pyriform polar capsules and a posterior end with very evident sporoplasm, measuring 8 ± 0.2 µmin length. The spore width was 5.8 ± 0.4 µm, with a thickness of 3.4 ± 0.2µm. The length of the polar capsules was 3.6 ± 0.3 µm and the width was 1.2 ± 0.2µm, with 6 to 7 turns of the polar filament. The divergences observed, regarding the morphometric and genetic structure of SSU rDNA, in relation to other Myxobolidae already described in the literature, confirm the description of the new species Myxobolus rangeli n. sp.

Atuação do cirurgião - dentista da atenção primária à saúde durante a pandemia da covid-19 / Role of primary health care dental surgeons during the COVID-19 pandemic / Papel de los cirujanos dentistas de atención primaria de salud durante la pandemia de COVID-19

Introdução:A Atenção Primária à Saúde é caracterizada como a porta de entrada preferencial do Sistema Único de Saúde, ocupando lugar central no serviço. Todavia, a pandemia da COVID-19 instigou a necessidade de mudanças naorganização do trabalho das equipes de saúde da atenção básica.Objetivo:Neste cenário, torna-se fundamental conhecer a atuação docirurgião-dentista da Atenção Primária à Saúdeno que se refere à oferta dos serviços odontológicos, alterações no processode trabalho e capacidade técnica de ação, frente às condições estabelecidas pela pandemiadaCOVID-19.Metodologia:Trata-se de um estudo de caráter transversal descritivo e abordagem quantitativa, desenvolvido em um município do interior baiano, com 29 cirurgiões-dentistas vinculados àatenção básica. Esses profissionais foram convidados a responder um questionário on-lineque abordou questões relativas ao desempenho dos cirurgiões-dentistas e a condução das ações de saúde bucal frente às medidas restritivas impostas pela pandemiada COVID-19.Resultados:Foi possível identificar que o processo de trabalho e acesso da população aos serviços de odontologia sofreram interferências, expressas através da limitação do acesso da população à saúde bucal, alterações nas técnicas terapêuticas e interrupção das ações em saúde de abrangência coletiva.Conclusões:O presente estudo apontou limitaçõesno processo de trabalho do cirurgião-dentista da Atenção Primária à Saúdedurante a pandemia da COVID-19, como a dificuldade para atuar em equipe interprofissional e atender as proposições estabelecidas pelaPolítica Nacional da Atenção Básica (AU).

Introduction:Primary Health Care is the preferred gateway to the Brazilian Unified Health System, occupying a central place in the service. However, the COVID-19 pandemic instigated the need for changes in the organization of work of primary care health teams.Objective:In this scenario, it is essential to know the performance of dental surgeons in terms ofdental care offer, changes in the work process and of their technical capacity for action, in the face of the conditions established by the pandemic.Methodology:This is a descriptive cross-sectional study with a quantitative approach developed in a city in the countryside of Bahia with 29 dental surgeons linked to primary care. These professionals were invited to answer an online questionnaire that addressed issues related to the performance of dental surgeons and the management of oral health actions in the face of the restrictive measures imposed by the COVID-19 pandemic.Results:It was possible to identify that the pandemic interfered with the work process and the population's access to dental services, expressed through the limitation of the population's access to oral health, changes in therapeutic techniques and interruption of collective health actions.Conclusions:The present study pointed out limitations in the work process of the primary care dentist during the COVID-19 pandemic, such as the difficulty in working in an interprofessional team and meeting the propositions established by the Brazilian National Primary Care Policy in Health (AU).

Introducción: La Atención Primaria de Salud se caracteriza por ser la puerta de entrada preferente al Sistema Único de SaludBrasileño, ocupando un lugar central en el servicio. Sin embargo, la pandemia de COVID-19 instigó la necesidad de cambios en la organización del trabajo de los equipos de salud de atención primaria.Objetivo: En este escenario, es fundamental conocer el desempeño del odontólogo en cuanto, oferta de cuidado dental, cambios enel procesode trabajo, ya su capacidad técnica de actuación, ante las condiciones establecidas por la pandemiaMetodología: Se trata de un estudio descriptivo transversal con enfoque cuantitativo, desarrollado en una ciudad del interior de Bahía, con 29 odontólogos vinculados a la atención primaria. Estos profesionales fueron invitados a responder un cuestionario en línea que abordaba cuestiones relacionadas con la actuación de los odontólogos y la realización de acciones de salud bucal frente a las medidas restrictivas impuestas por la pandemia de la COVID-19.Resultados: Con los resultados fue posible identificar que el proceso de trabajo y el acceso de la población a los servicios odontológicos sufrieron interferencias, expresadas a través de la limitación del acceso de la población a la salud bucal, cambios en las técnicas terapéuticas e interrupción de las acciones de salud colectiva. Conclusiones: El presente estudio señaló limitaciones en el proceso de trabajo del cirujano dentista de la atención en lasaluddurante la pandemia de COVID-19, como la dificultad para trabajar enequipo interprofesional y cumplir con las proposiciones establecidas por la Política Brasileña de Atención Primaria en Salud (AU).

Acanthus mollis Formulations for Transdermal Delivery: From Hydrogels to Emulsions.

Topical formulations of Acanthus mollis L. leaf and the optimization of the release of their active compounds and their topical bioavailability were investigated for the first time. In vitro, the release of active compounds from three formulations-an oil-in-water cream and two hydrogels (Carbopol 940 and Pluronic F-127)-was determined using Franz diffusion cells. Detection and quantification of the compounds was performed via high-performance liquid chromatography with a photodiode array (HPLC-PDA). DIBOA, a bioactive compound of this medicinal plant, exhibited release kinetics of the Weibull model for the Carbopol and Pluronic F-127 formulation, identifying it as a potential active agent to optimize the topical distribution of the formulations. The implications extend to applications in inflammation treatment and tyrosinase inhibition, suggesting that it can make a significant contribution to addressing skin conditions, including melanoma and various inflammatory diseases.

Upconversion rare Earths nanomaterials applied to photodynamic therapy and bioimaging.

Light-based therapies and diagnoses including photodynamic therapy (PDT) have been used in many fields of medicine, including the treatment of non-oncological diseases and many types of cancer. PDT require a light source and a light-sensitive compound, called photosensitizer (PS), to detect and destroy cancer cells. After absorption of the photon, PS molecule gets excited from its singlet ground state to a higher electronically excited state which, among several photophysical processes, can emit light (fluorescence) and/or generate reactive oxygen species (ROS). Moreover, the biological responses are activated only in specific areas of the tissue that have been submitted to exposure to light. The success of the PDT depends on many parameters, such as deep light penetration on tissue, higher PS uptake by undesired cells as well as its photophysical and photochemical characteristics. One of the challenges of PDT is the depth of penetration of light into biological tissues. Because photon absorption and scattering occur simultaneously, these processes depend directly on the light wavelength. Using PS that absorbs photons on "optical transparency windows" of biological tissues promises deeper penetration and less attenuation during the irradiation process. The traditional PS normally is excited by a higher energy photon (UV-Vis light) which has become the Achilles' heel in photodiagnosis and phototreatment of deep-seated tumors below the skin. Thus, the need to have an effective upconverter sensitizer agent is the property in which it absorbs light in the near-infrared (NIR) region and emits in the visible and NIR spectral regions. The red emission can contribute to the therapy and the green and NIR emission to obtain the image, for example. The absorption of NIR light by the material is very interesting because it allows greater penetration depth for in vivo bioimaging and can efficiently suppress autofluorescence and light scattering. Consequently, the penetration of NIR radiation is greater, activating the biophotoluminescent material within the cell. Thus, materials containing Rare Earth (RE) elements have a great advantage for these applications due to their attractive optical and physicochemical properties, such as several possibilities of excitation wavelengths - from UV to NIR, strong photoluminescence emissions, relatively long luminescence decay lifetimes (µs to ms), and high sensitivity and easy preparation. In resume, the relentless search for new systems continues. The contribution and understanding of the mechanisms of the various physicochemical properties presented by this system is critical to finding a suitable system for cancer treatment via PDT.

Synergistic Effect of DIBOA and Verbascoside from Acanthus mollis Leaf on Tyrosinase Inhibition.

Overexpression of melanin contributes to darkening of plant and fruit tissues and skin hyperpigmentation, leading to melasma or age spots. Although melanin biosynthesis is complex and involves several steps, a single enzyme known as tyrosinase is key to regulating this process. The melanogenesis pathway is initiated by oxidation of the starting material l-tyrosine (or l-DOPA) to dopaquinone by tyrosinase; the resulting quinone then serves as a substrate for subsequent steps that eventually lead to production of melanin. Medicinal plants are considered a good source of tyrosinase inhibitors. This study investigated the tyrosinase inhibitory activity of A. mollis leaf extracts and their phytochemicals. Significant activity was verified in the ethanol extract -EEt (IC50 = 1.21 µg/mL). Additionally, a kinetic study showed that this tyrosinase inhibition occurs by DIBOA (2,4-dihydroxy-1,4-benzoxazin-3-one) and verbascoside contribution through a non-competitive reaction mechanism. A synergistic effect on tyrosinase inhibition was observed in the binary combination of the compounds. In conclusion, both EEt and a mixture of two of its phytochemicals can be effective tyrosinase inhibitors and can be used as a bleaching agent for cosmetic formulations in the future.

Forest Biomass as a Promising Source of Bioactive Essential Oil and Phenolic Compounds for Alzheimer's Disease Therapy.

Alzheimer's disease (AD) is the most common neurodegenerative disorder affecting elderly people worldwide. Currently, there are no effective treatments for AD able to prevent disease progression, highlighting the urgency of finding new therapeutic strategies to stop or delay this pathology. Several plants exhibit potential as source of safe and multi-target new therapeutic molecules for AD treatment. Meanwhile, Eucalyptus globulus extracts revealed important pharmacological activities, namely antioxidant and anti-inflammatory properties, which can contribute to the reported neuroprotective effects. This review summarizes the chemical composition of essential oil (EO) and phenolic extracts obtained from Eucalyptus globulus leaves, disclosing major compounds and their effects on AD-relevant pathological features, including deposition of amyloid-ß (Aß) in senile plaques and hyperphosphorylated tau in neurofibrillary tangles (NFTs), abnormalities in GABAergic, cholinergic and glutamatergic neurotransmission, inflammation, and oxidative stress. In general, 1,8-cineole is the major compound identified in EO, and ellagic acid, quercetin, and rutin were described as main compounds in phenolic extracts from Eucalyptus globulus leaves. EO and phenolic extracts, and especially their major compounds, were found to prevent several pathological cellular processes and to improve cognitive function in AD animal models. Therefore, Eucalyptus globulus leaves are a relevant source of biological active and safe molecules that could be used as raw material for nutraceuticals and plant-based medicinal products useful for AD prevention and treatment.

A review of the ethnomedicinal uses, chemistry, and pharmacological properties of the genus Acanthus (Acanthaceae).

ETHNOPHARMACOLOGICAL RELEVANCE: The Acanthus genus belongs to the Acanthaceae family, and its species are distributed in all continents, mainly in tropical and subtropical regions. Several traditional applications are referred to, but few scientific studies validate them. Despite this, studies in animal models corroborate some of its uses in folk medicine, such as anticancer, antidiabetic, anti-inflammatory, and antinociceptive, which encourages the research on plants of this genus. AIM OF THE REVIEW: To our knowledge, this document is the first comprehensive review study that provides information on the geographic distribution, botanical characteristics, ethnomedicinal uses, phytochemicals, and pharmacological activities of some Acanthus species to understand the correlation between traditional uses, phytochemical, and pharmacological activities, providing perspectives for future studies. RESULTS: In traditional medicine, Acanthus species are mainly used for diseases of respiratory, nervous and reproductive system, gastrointestinal and urinary tract, and skin illness. The most used species are A. montanus, A. ilicifolius, and A. ebracteatus. Chemical compounds (125) from different chemical classes were isolated and identified in seven species, mainly from A. ilicifolius, about 80, followed by A. ebracteatus and A. montanus, appearing with a slightly lower number with fewer phytochemical profile studies. Isolated phytoconstituents have been mainly alkaloids, phenylpropanoid glycosides, and phenylethanoids. In addition, aliphatic glycosides, flavonoids, lignan glycosides, megastigmane derivatives, triterpenoids, steroids, fatty acids, alcohols, hydroxybenzoic acids, simple phenols were also cited. Scientific studies from Acanthus species extracts and their phytoconstituents support their ethnomedical uses. Antimicrobial activity that is the most studied, followed by the antioxidant, anti-inflammatory, and anticancer properties, underlie many Acanthus species activities. A. dioscoridis, A. ebracteatus, A. hirsutus, A. ilicifolius, A. mollis, A. montanus, and A. polystachyus have studies on these activities, A. ilicifolius being the one with the most publications. Most studies were essentially performed in vitro. However, the anticancer, antidiabetic, anti-inflammatory and antinociceptive properties have been studied in vivo. CONCLUSION: Acanthus species have remarkable phytoconstituents with different biological activities, such as antioxidant, antimicrobial, anti-inflammatory, antinociceptive, hepatoprotective, and leishmanicidal, supporting traditional uses of some species. However, many others remain unexplored. Future studies should focus on these species, especially pharmacological properties, toxicity, and action mechanisms. This review provides a comprehensive report on Acanthus genus plants, evidencing their therapeutic potential and prospects for discovering new safe and effective drugs from Acanthus species.

Chemical Composition and Effect against Skin Alterations of Bioactive Extracts Obtained by the Hydrodistillation of Eucalyptus globulus Leaves.

Eucalyptus globulus is planted extensively for pulp, paper and wood production. Although bioactive compounds obtained from its biomass are used as cosmetics ingredients, the skin effects were not yet fully explored. In order to fill this gap, this work aimed to study the protective effect against skin damage provided by the essential oil (EO) obtained from the hydrodistillation of Eucalyptus globulus leaves, and by an extract obtained from the hydrodistillation residual water (HRW). The major compound identified in the EO was 1,8-Cineole, and the phenolic acids in the HRW included gallic acid as the main phenolic constituent. Moreover, non-toxic EO and HRW concentrations were shown to have anti-aging skin effects in vitro, decreasing age-related senescence markers, namely ß-galactosidase and matrix metalloproteinases activation, as well as collagen type 1 upregulation. In addition, EO and HRW were found to exhibit depigmenting effects by inhibiting tyrosinase and melanin production, along with potent anti-inflammatory properties. Furthermore, the absence of skin irritation and sensitization in cells exposed to EO and HRW revealed the safety of both extracts for topical use. Taken together, these results highlight the beneficial effects of extracts obtained from Eucalyptus globulus biomass for skin aesthetic and health purposes, which should be explored deeply for the prediction of future pharmaceutical and dermocosmetics industrial applications.

Utrastructure and molecular phylogeny of the myxozoan Kudoa ocellatus n. sp. (Myxozoa: Kudoidae), a parasite of the Oscar, Astronotus ocellatus (Agassiz, 1831; Teleostei: Cichlidae), a fish from northern Brazil.

Kudoa ocellatus n. sp. was found in the musculature of Astronotus ocelattus (Agassiz, 1831) from the Arari River on Marajó Island in Pará, Brazil. The new species forms pseudocysts in the epaxial and hypaxial musculature composed of various spores that are pseudoquadrate in the apical view. In the lateral view, the spores were triangular or pyramidal. In the lateral view, the spores were 46 ± 0.11 µm (4.5-4.8) in length and 6.6 ± 0.3 µm (6.2-7.2) in width, with four pyriform polar capsules of equal size that measured 2.0 ± 0.16 µm (1.8-2.2) in length and 1.5 ± 0.18 µm (1.3-1.8) in width. Based on the partial (1418 bps) sequence of the SSU rDNA gene, Kudoa ocellatus n. sp. was distinct from all the other Kudoa species deposited in GenBank. The phylogenetic Bayesian Inference and P distance placed the new species together with the other Kudoa species that parasitize freshwater Amazonian fish. The morphological evidence, together with the SSU rDNA gene sequence, supported the description of Kudoa ocellatus n. sp., a distinct new species of the genus, which parasitizes a freshwater Amazonian cichlid.

Blueberry Counteracts Prediabetes in a Hypercaloric Diet-Induced Rat Model and Rescues Hepatic Mitochondrial Bioenergetics.

The paramount importance of a healthy diet in the prevention of type 2 diabetes is now well recognized. Blueberries (BBs) have been described as attractive functional fruits for this purpose. This study aimed to elucidate the cellular and molecular mechanisms pertaining to the protective impact of blueberry juice (BJ) on prediabetes. Using a hypercaloric diet-induced prediabetic rat model, we evaluated the effects of BJ on glucose, insulin, and lipid profiles; gut microbiota composition; intestinal barrier integrity; and metabolic endotoxemia, as well as on hepatic metabolic surrogates, including several related to mitochondria bioenergetics. BJ supplementation for 14 weeks counteracted diet-evoked metabolic deregulation, improving glucose tolerance, insulin sensitivity, and hypertriglyceridemia, along with systemic and hepatic antioxidant properties, without a significant impact on the gut microbiota composition and related mechanisms. In addition, BJ treatment effectively alleviated hepatic steatosis and mitochondrial dysfunction observed in the prediabetic animals, as suggested by the amelioration of bioenergetics parameters and key targets of inflammation, insulin signaling, ketogenesis, and fatty acids oxidation. In conclusion, the beneficial metabolic impact of BJ in prediabetes may be mainly explained by the rescue of hepatic mitochondrial bioenergetics. These findings pave the way to support the use of BJ in prediabetes to prevent diabetes and its complications.

Contaminação microbiológica de resinas compostas utilizadas em uma clínica-escola de Odontologia / Microbiological contamination of composite resins used in a Dental school clinic

O objetivo desse estudofoi verificar a contaminação microbiológica deresinas compostas utilizadas em uma clínica-escola de Odontologia. Trata-se de uma pesquisa experimental/laboratorial, na qual foramcoletadas amostras de 10 bisnagas de resina composta, sendo uma delas o controle negativo. Porções de resina composta contidas no interior das bisnagas foram coletadas e mergulhadas em tubos de ensaio contendo caldo nutriente eposterior semeadura em placas e coloraçãopara caracterizar as colônias e observar bactérias e fungos. Todas as amostras apresentaram contaminação, inclusive o tubo contendo meio de cultura utilizado como controle de manuseio do experimento. Essas contaminações podem ter relação com as falhas dos meios de biossegurança empregados na clínica-escola e com os métodos de transporte e armazenamento das bisnagas de resina composta. Portanto, existe a necessidade de conscientização dos estudantese docentespara a adoção de medidas de biossegurança específicas para o manuseio das resinas compostas (AU).

The aim of this study was to verify the microbiological contamination in composite resins used at one Dental school clinic. This is an experimental/laboratory study, in which samples of 10 tubes of composite resin were collected, one of which was the negative control. Portions of composite resin inside the tubes were collected and dipped in test tubes containing nutrient broth for subsequent seeding on to plates and staining for the characterization of bacterial and fungal colonies. All samples revealed contamination, including the tube containing culture medium used as control for handling the experiment. These contaminations may be related to failures in biosafety measures employed in theDental school clinic and to the transport and storage methods for the tubes of composite resin. Therefore, it is necessary to raise awareness among students and teachers to adopt specific biosafety measures for the handling of composite resins (AU).

Moving forward: Exploring the role of interference on prospective memory deactivation.

Recent prospective memory (PM) studies have shown that an intention may be erroneously executed despite no-longer-needed (i.e., commission errors), especially under demanding ongoing activities. In the current study, we examined whether PM deactivation benefits from a retroactive interference mechanism. For this, we set up a procedure in which participants are first asked to perform a PM task which is critically declared finished afterwards. Next, they encoded a new and dissimilar PM intention to accomplish later (Experiment 1) or performed filler tasks with increased working memory difficulty levels (Experiment 2). Lastly, all participants encountered several (but irrelevant) PM cues. Together, our findings provide evidence that the efficiency of the deactivation process can be modulated by encoding novel and dissimilar PM tasks and by the type of processing after intention completion. These findings are discussed in terms of strategic or spontaneous retrieval processes and linked to a retroactive interference mechanism which helps to overwrite or deteriorate the old-PM task representation.

From retrospective to prospective memory research: a framework for investigating the deactivation of intentions.

The definition of episodic memory has evolved into a multifaceted concept that gathered great attention in several research areas in psychology and neuroscience. Prospective memory (PM), or the ability to remember to perform delayed intentions at a later moment in the future, represents one side of this capacity for which that has been a growing interest. In this review, we examined a counterintuitive finding: PM intentions may persist and affect our behaviour despite successful goal attainment and task completion, which in daily life may be as serious as taking medication twice. This review aims to elucidate the existing knowledge and identify some unresolved questions concerning this specific memory failure. Therefore, this review provides an overview of the uprising research dedicated to both PM omission and commission errors, including an analysis of its definitions, of the current theoretical approaches of PM retrieval, and the main procedures used in this field to offer an integrative perspective on this topic. Finally, the last section is devoted to discussing future directions to test the predictions of our suggested theoretical explanations for PM deactivation. This might be an avenue for research that is likely to extend our understanding of episodic memory's usefulness in everyday life.

Myxobolus freitasi n. sp. (Myxozoa: Bivalvulida), a parasite of the brain of the electric knifefish in the Brazilian Amazon region.

A total of 30 specimens of the Amazonian electric knifefish, Brachyhypopomus beebei Schultz, 1944 (Gymnotiformes: Hypopomidae), were collected from the Peixe-Boi River in the state of Pará, Brazil (1°06'59" S; 47°18'26" W). Fragments of the brain tissue were extracted for analysis via optical microscopy, and 18 specimens (60%) presented microparasites of the genus Myxobolus, with unequal capsules. The spores were 18.6 µm (17.7-19.8 µm) long and 8.6 µm (8.4-9.0 µm) wide; the largest polar capsule was 13.0 µm (12.4-13.4 µm) long and 5.6 µm (5.3-6.0 µm) wide, and the smallest capsule was 5.0 µm (4.5-5.3 µm) long and 2.5 µm (2.3-2.6 µm) wide. Infected brain fragments were extracted for histological processing and staining with hematoxylin-eosin and Ziehl-Neelsen. Some fragments were conserved in ethanol for molecular genetics analysis. A partial sequence of the 18S DNA gene was obtained from the spores, which did not correspond to any other sequences deposited in GenBank, although it did form a clade with other Myxobolus parasites of the nervous system. The morphological data, together with molecular phylogeny, supported the designation of a new species Myxobolus freitasi n. sp.

Determination of chemical structure and anti-Trypanosoma cruzi activity of extracts from the roots of Lonchocarpus cultratus (Vell.) A.M.G. Azevedo & H.C. Lima.

Trypanosoma cruzi is the agent of Chagas disease, an infection that affects around 8 million people worldwide. The search for new anti-T. cruzi drugs are relevant, mainly because the treatment of this disease is limited to two drugs. The objective of this study was to investigate the trypanocidal and cytotoxic activity and elucidate the chemical profile of extracts from the roots of the Lonchocarpus cultratus. Roots from L. cultratus were submitted to successive extractions with hexane, dichloromethane, and methanol, resulting in LCH, LCD, and LCM extracts, respectively. Characterization of extracts was done using 1H-RMN, 13C-RMN, CC and TLC. Treatment of T. cruzi forms (epimastigotes, trypomastigotes, and amastigotes) with crescent concentrations of LCH, LCD, and LCM was done for 72, 48, and 48 h, respectively. After this, the percentage of inhibition and IC50/LC50 were calculated. Benznidazole was used as a positive control. Murine macrophages were treated with different concentrations of both extracts for 48 h, and after, the cellular viability was determined by the MTT method and CC50 was calculated. The chalcones derricin and lonchocarpine were identified in the hexane extract, and for the first time in the genus Lonchocarpus, the presence of a dihydrolonchocarpine derivative was observed. Other chalcones such as isocordoin and erioschalcone B were detected in the dichloromethane extract. The dichloromethane extract showed higher activity against all tested forms of T. cruzi than the other two extracts, with IC50 values of 10.98, 2.42, and 0.83 µg/mL, respectively; these values are very close to those of benznidazole. Although the dichloromethane extract presented a cytotoxic effect against mammalian cells, it showed selectivity against amastigotes. The methanolic extract showed the lowest anti-T. cruzi activity but was non-toxic to peritoneal murine macrophages. Thus, the genus Lonchocarpus had demonstrated in the past action against epimastigotes forms of T. cruzi but is the first time that the activity against infective forms is showed, which leading to further studies with in vivo tests.

Myxobolus freitasi n. sp. (Myxozoa: Bivalvulida), a parasite of the brain of the electric knifefish in the Brazilian Amazon region / Myxobolus freitasi n. sp. (Myxozoa: Bivalvulida), parasita do cérebro do peixe-faca elétrico na Amazônia brasileira

Abstract A total of 30 specimens of the Amazonian electric knifefish, Brachyhypopomus beebei Schultz, 1944 (Gymnotiformes: Hypopomidae), were collected from the Peixe-Boi River in the state of Pará, Brazil (1°06'59" S; 47°18'26" W). Fragments of the brain tissue were extracted for analysis via optical microscopy, and 18 specimens (60%) presented microparasites of the genus Myxobolus, with unequal capsules. The spores were 18.6 µm (17.7-19.8 µm) long and 8.6 µm (8.4-9.0 µm) wide; the largest polar capsule was 13.0 µm (12.4-13.4 µm) long and 5.6 µm (5.3-6.0 µm) wide, and the smallest capsule was 5.0 µm (4.5-5.3 µm) long and 2.5 µm (2.3-2.6 µm) wide. Infected brain fragments were extracted for histological processing and staining with hematoxylin-eosin and Ziehl-Neelsen. Some fragments were conserved in ethanol for molecular genetics analysis. A partial sequence of the 18S DNA gene was obtained from the spores, which did not correspond to any other sequences deposited in GenBank, although it did form a clade with other Myxobolus parasites of the nervous system. The morphological data, together with molecular phylogeny, supported the designation of a new species Myxobolus freitasi n. sp.

Resumo Um total de 30 espécimes do peixe-faca elétrico da Amazônia, Brachyhypopomus beebei Schultz, 1944 (Gymnotiformes: Hypopomidae), foram coletados no rio Peixe-Mani, no estado do Pará, Brasil (1 ° 06'59 "S; 47 ° 18 ' 26 "W). Fragmentos de tecido cerebral foram extraídos para análise em microscopia óptica, sendo que 18 espécimes (60%) apresentavam microparasitos do gênero Myxobolus, com cápsulas desiguais. Os esporos apresentavam 18,6 µm (17,7-19,8 µm) de comprimento e 8,6 µm (8,4-9,0 µm) de largura; a maior cápsula polar tinha 13,0 µm (12,4-13,4 µm) de comprimento e 5,6 µm (5,3-6,0 µm) de largura, e a menor cápsula tinha 5,0 µm (4,5-5,3 µm) de comprimento e 2,5 µm (2,3-2,6 µm) de largura. Fragmentos cerebrais infectados foram extraídos para processamento histológico e coloração com hematoxilina-eosina e Ziehl-Neelsen. Alguns fragmentos foram conservados em etanol para análise genética molecular. Dos esporos, foi obtida uma sequência parcial do gene 18S do DNA, que não correspondeu a nenhuma outra sequência depositada no GenBank, embora tenha formado um clado com outros parasitas do gênero Myxobolus do sistema nervoso. Os dados morfológicos, juntamente com a filogenia molecular, apoiaram a designação de uma nova espécie Myxobolus freitasi n. sp.

How Does Performing Demanding Activities Influence Prospective Memory? A Systematic Review.

This paper is the first systematic review on the role of ongoing task load in prospective remembering, which was conducted according to the Preferred Reporting Items for Systematic Reviews and Meta-Analysis (PRISMA). Forty articles published between 1995 and 2020 were included. They evaluated prospective memory (PM) performance (i.e., the ability to remember to execute a delayed intention) in adult samples aged between 19 and 50 years old when the PM cue appeared under cognitively demanding conditions. The results revealed that people are more likely to fail to remember to perform a delayed intention at the appropriate circumstances or time in the future when their cognitive resources are taxed by demanding ongoing activities. We conclude the review by highlighting that the degree of working memory and executive resources seems to account for some of the discrepant findings and by proposing directions for future research.

When we must forget: the effect of cognitive load on prospective memory commission errors.

Recent studies consistently show that prospective memory (PM) intentions are not always deactivated when no-longer needed and might be erroneously performed upon encountering the once relevant cue - termed PM commission errors. However, empirical evidence on the potential mechanisms that might lead to this kind of memory failure remains mostly unexplored. This study aimed to investigate the influence of the ongoing task demands on PM deactivation of non-performed intentions. Younger adults, except for those in the no-PM condition, were asked to perform a PM task and were then told that the intention was finished. Later, they perform a lexical decision task with some trials containing (irrelevant) PM cues while simultaneously carrying out a counting recall task with two levels of difficulty. The results showed a higher risk of PM commission errors under moderate cognitive load (74%) as compared to the no-load condition (40%). Results also show that commission error risk did not increase in the high-load (54%) compared with the moderate-load condition. Furthermore, comparisons of the ongoign task performance between the no-PM condition and the other conditions with a PM task requirement support that commission errors might arise from a spontaneous PM retrieval. The implications of these findings are discussed within the dual-mechanisms account.

Manutenção do aleitamento materno no retorno ao trabalho / Maintenance of breastfeeding in the return to work / Mantenimiento del alcance materno en el retorno al trabajo

Pesquisa foi motivada a investigar como as mulheres trabalhadoras realizava a manutenção do aleitamento materno ao retornar de seu trabalho, que teve como objetivo descrever se havia dificuldades e estratégias realizadas para a manutenção do aleitamento. O método análise dos dados pelo referencial de Bardin, realizada entre os meses de setembro à outubro de 2017, com vinte mulheres entre 19 a 39 anos de idade cadastradas e atendidas na unidade de estratégia saúde da família do município do Rio de Janeiro, sendo produzidas duas categorias: Paradoxo da rede de apoio; Ausência do Enfermeiro no trabalho multiprofissional. Resultado obteve como desmame precoce havendo a introdução de leite pasteurizado como consequência. Conclui-se que há insegurança para a realização de ações de continuidade de aleitamento materno após o retorno ao trabalho.(AU)

The research was motivated to investigate the hard-working women accomplished the maintenance of the maternal breast feeding when returning of his / her work, that he / she had an objective described if there were difficulties and strategies accomplished for the maintenance of the breast feeding. The methodology analysis of the data for the reference of Bardin, conducted between the months of September to October of 2017, twenty women among 19 to 39 years of age registered and assisted in the unit of strategy. municipal district of Rio de Janeiro, being produced two categories: Paradox of the support net; Absence of the Nurse in the multi-professional work. Result obtained how it weans precocious having the introduction of milk pasteurized as consequence. It is ended that there is insecurity for the accomplishment of actions of continuity of maternal breast feeding after the return to the work.(AU)

La investigación fue motivada a investigar cómo las mujeres trabajadoras realizaban el mantenimiento de la lactancia materna al retornar de su trabajo, que tuvo como objetivo describir si había dificultades y estrategias realizadas para el mantenimiento de la lactancia. La metodología análisis de los datos por el referencial de Bardin, realizada entre los meses de septiembre a octubre de 2017, veinte mujeres entre 19 a 39 años de edad registradas y atendidas en la unidad de estrategia salud de la familia del municipio de Río de Janeiro En enero, se producirá dos categorías: Paradoja de la red de apoyo; Ausencia del enfermero en el trabajo multiprofesional. Resultado obtenido como destete precoz habiendo la introducción de leche pasteurizada como consecuencia. Se concluye que hay inseguridad para la realización de acciones de continuidad de lactancia materna después del retorno al trabajo.(AU)